They also form part of a complex and heterogeneous tissue along with multiple cell types including myoepithelial, stromal and immune cells, making it difficult to precisely assign gene expression to the different cell types. Thus, it is important to distinguish which cell populations play specific functions, and to obtain relevant expression profile data reflecting the in vivo state in order to recognize differences in these cell fractions and to obtain reliable transcriptomic results1. Mammary epithelial cells are normally subject to turnover and are replaced by new cells2.
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